GHK-Cu (50mg Vial)

GHK-Cu is a bioactive complex formed by the tripeptide GHK bound to copper (II). It is a molecule naturally present in the body, known for supporting cellular renewal, tissue repair, and skin integrity. With age, its levels tend to decline, which has driven its use in advanced aesthetic and regenerative protocols.

Key Benefits

Firmer, more elastic, denser skin, with a more “filled” and healthy appearance.

Improved texture and radiance, with softening of fine lines and a more even look.

Support for skin repair and recovery, helping maintain cutaneous integrity after external stressors.

Cellular protection, helping regulate inflammatory processes and the impact of oxidative stress.

Overall improved skin quality, supporting a revitalised and rejuvenated appearance throughout the protocol.

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GHK-Cu is a bioactive complex formed by the tripeptide GHK bound to copper (II). It is a molecule naturally present in the body, known for supporting cellular renewal, tissue repair, and skin integrity. With age, its levels tend to decline, which has driven its use in advanced aesthetic and regenerative protocols.

Key Benefits

Firmer, more elastic, denser skin, with a more “filled” and healthy appearance.

Improved texture and radiance, with softening of fine lines and a more even look.

Support for skin repair and recovery, helping maintain cutaneous integrity after external stressors.

Cellular protection, helping regulate inflammatory processes and the impact of oxidative stress.

Overall improved skin quality, supporting a revitalised and rejuvenated appearance throughout the protocol.

Technical Details

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RECONSTITUTION, DOSING AND STORAGE OF PEPTIDES IN A VIAL
(General guidance – not peptide-specific)

1. INTRODUCTION
Peptides supplied in a vial (a glass vial containing lyophilised powder) are not ready for immediate use.
Before administration, it is mandatory to carry out reconstitution, which is the process of converting the lyophilised powder into a sterile liquid solution.
Correct reconstitution is essential to ensure:
Safe use
Peptide stability
Accurate dosing
Protocol effectiveness
⚠️ Incorrect preparation may result in inaccurate dosing and reduced effectiveness.

2. REQUIRED MATERIALS
Vial containing the lyophilised peptide
Bacteriostatic Water
    Contains approximately 0.9% benzyl alcohol
    Helps inhibit bacterial growth
Insulin syringe (100 units = 1 mL)
Sterile needle
Clean, hygienic environment

3. WHAT RECONSTITUTION IS
Reconstitution is the controlled addition of bacteriostatic water to the lyophilised powder, creating a liquid solution suitable for subcutaneous use.

4. GENERAL RULE FOR CALCULATING CONCENTRATION
Basic formula:
Amount of peptide (mg) ÷ Volume of water (mL) = Concentration (mg/mL)
This concentration is used to calculate the correct dose in the syringe.

5. CORRECT RECONSTITUTION TECHNIQUE
⚠️ IMPORTANT: PEPTIDES ARE FRAGILE MOLECULES
Recommended procedure:

  1. Draw the desired volume of bacteriostatic water into the syringe.
  2. Insert the needle into the vial.
  3. Allow the water to run slowly down the inner wall of the vial.
  4. Do not direct the stream straight onto the powder.
  5. Do not shake the vial.

Gently roll the vial between your fingers until fully dissolved.
**Vigorous shaking may cause peptide degradation.

6. CONVERSION FOR AN INSULIN SYRINGE
 1 mL = 100 units
The amount of peptide per unit depends on the final concentration after reconstitution.
Always calculate dosing based on the concentration (mg/mL).

7. STORAGE AFTER RECONSTITUTION
Keep refrigerated between 2°C and 8°C
Do not freeze
Keep the vial tightly sealed
Protect from light, especially for photosensitive peptides

8. FINAL CONSIDERATIONS

  • Always follow the correct preparation technique
  • Use bacteriostatic water only
  • Maintain strict accuracy in dose calculations
  • Use should be carried out under professional supervision

 

Siméon A et al., 2000 (Journal of Investigative Dermatology) — describes modulation of extracellular matrix components and cites clinical evidence in wound healing.
GHK-Cu 50 mg – Reconstitution and Dosage
What is GHK-Cu
GHK-Cu (Glycyl-L-Histidyl-L-Lysine Copper) is a copper-binding bioactive peptide widely studied for its role in tissue regeneration, collagen synthesis, wound healing, skin health and hair restoration.
Reconstitution – 50 mg Vial
Materials required
•GHK-Cu 50 mg lyophilised vial
•Sterile bacteriostatic water
•Sterile syringe
Recommended volume
To facilitate clinical dosing:
Add 5 mL of bacteriostatic water
Final concentration
50 mg ÷ 5 mL = 10 mg/mL
Insulin syringe equivalence (100 IU)
•1 mL = 100 IU
•Concentration: 0.1 mg per IU
Desired dose Volume Units (IU)
1 mg 0.1 mL 10 IU
2 mg 0.2 mL 20 IU
2.5 mg 0.25 mL 25 IU
3 mg 0.3 mL 30 IU
Recommended Dosage (Technical Protocols)
Systemic use – Subcutaneous
Protocols commonly used in regenerative and aesthetic medicine:
Standard dose: 2 to 2.5 mg per administration
•Frequency: 2 to 3 times per week
•Initial duration: 6 to 8 weeks
•Maintenance: 1 to 2 administrations per week, according to clinical response
Injection sites
•Abdomen (subcutaneous)
•Lateral thigh
•Flank area
Local use (tissue regeneration or aesthetic applications)
•1 to 2.5 mg per treated area
•1 to 2 times per week
Indications commonly include:
•Skin rejuvenation
•Alopecia support
•Tissue repair and healing
Storage
•After reconstitution: store refrigerated at 2°C to 8°C
•Do not freeze
•Protect from light
•Shelf life after reconstitution: up to 30 days when prepared with bacteriostatic water
Safety and Considerations
•Intended for use by qualified professionals only
•For clinical research, regenerative or aesthetic applications in accordance with local regulations
•Do not exceed recommended dosages without professional supervision

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